Generation of a strong mutator phenotype in yeast by imbalanced base excision repair
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چکیده
منابع مشابه
Generation of a strong mutator phenotype in yeast by imbalanced base excision repair.
Increased spontaneous mutation is associated with increased cancer risk. Here, by using a model system, we show that spontaneous mutation can be increased several hundred-fold by a simple imbalance between the first two enzymes involved in DNA base excision repair. The Saccharomyces cerevisiae MAG1 3-methyladenine (3MeA) DNA glycosylase, when expressed at high levels relative to the apurinic/ap...
متن کاملHypoxia provokes base excision repair changes and a repair-deficient, mutator phenotype in colorectal cancer cells.
UNLABELLED Regions of acute and chronic hypoxia exist within solid tumors and can lead to increased rates of mutagenesis and/or altered DNA damage and repair protein expression. Base excision repair (BER) is responsible for resolving small, non-helix-distorting lesions from the genome that potentially cause mutations by mispairing or promoting DNA breaks during replication. Germline and somatic...
متن کاملDNA Damage and Repair Hypoxia Provokes Base Excision Repair Changes and a Repair-Deficient, Mutator Phenotype in Colorectal Cancer Cells
Regions of acute and chronic hypoxia exist within solid tumors and can lead to increased rates ofmutagenesis and/ or altered DNA damage and repair protein expression. Base excision repair (BER) is responsible for resolving small, non–helix-distorting lesions from the genome that potentially cause mutations by mispairing or promoting DNA breaks during replication. Germline and somatic mutations ...
متن کاملImbalanced base excision repair increases spontaneous mutation and alkylation sensitivity in Escherichia coli.
Inappropriate expression of 3-methyladenine (3MeA) DNA glycosylases has been shown to have harmful effects on microbial and mammalian cells. To understand the underlying reasons for this phenomenon, we have determined how DNA glycosylase activity and substrate specificity modulate glycosylase effects in Escherichia coli. We compared the effects of two 3MeA DNA glycosylases with very different s...
متن کاملDysregulated Expression and Sub cellular Localization of Base Excision Repair (BER) Pathway Enzymes in Gallbladder Cancer
Base excision repair (BER) pathway is one of the repair systems that have an impact on the radiotherapy and chemotherapy for the cancer patients. The molecular pathogenesis of gallbladder cancer is not known extensively. In the present study we investigated whether the expression of AP endonuclease 1 (APE1) and DNA polymerase β (DNA pol β), key enzymes of BER pathway has any clinical ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1998
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.95.17.9997